Electronic Journal of Liver Tumor ›› 2022, Vol. 9 ›› Issue (1): 36-40.

• Original article • Previous Articles     Next Articles

Role of BAI1 gene in anti-angiogenesis of hepatic carcinoma

Zhu Qing1, Wei Zhewen2, Wang Xiaobing3,*, Cai Jianqiang2,*   

  1. 1. Department of Clinical Laboratory Diagnostics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;
    2. Department of Hepatobiliary Surgery, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China;
    3. State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China
  • Received:2021-11-12 Online:2022-03-31 Published:2022-10-28

Abstract: Objective:To explore the role of BAI1 gene in anti-angiogenesis of hepatic carcinoma.
Method:The BAI1 gene knockout PLC/PRF/5 cell line was constructed using CRISPR/Cas9 technology, and the knockout results were verified by Sanger sequencing and Western blotting. The CCK8 method was used to detect the effect of BAI1 gene knockout on the proliferation of liver cancer cell lines. The PLC/PRF/5 cell line was co-cultured with human umbilical vein endothelial cells(HUVEC) to detect the effect of BAI1 gene expression on the ability of cell tube formation. Transplanted tumor model was used to study the effect of BAI1 gene expression on liver cancer growth and tumor angiogenesis in vivo.
Result:Sanger sequencing and Western blotting showed that BAI1 gene was successfully knocked out. The proliferation of PLC/PRF/5 cells was significantly accelerated and the number of tube was increased significantly with BAI1 gene deficiency. The tumor growth was inhibited in vivo with BAI1 gene overexpression, and the positive expression of CD31 was decreased.
Conclusion:The BAI1 gene knockout PLC/PRF/5 cell line was successfully constructed using CRISPR/Cas9 technology. BAI1 gene deficiency promote the proliferation of liver cancer cells and angiogenesis.

Key words: BAI1 gene, Anti-angiogenesis, Hepatic carcinoma