Establishment of a rat model: associating liver partition and portal vein ligation for staged hepatectomy with hepatic fibrosis

Abstract

Abstract: Objective:To establish the liver fibrosis model of associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) by carbon tetrachloride (CCl₄) and ethanol, and to explore the feasibility and safety of ALPPS in rats with liver fibrosis.
Method:Totally 40 male SD rats were randomly allocated by lot into two groups with 20 rats in each group. The control group was the blank group, which was routinely fed and injected with olive oil for 4 weeks, and the liver fibrosis group was CCl₄ subcutaneous injection + forced drinking of 10% ethanol for 4 weeks. 5 rats in the liver fibrosis group and the control group were stained with Sirius red to judge the degree of liver fibrosis. The remaining 15 rats underwent ALPPS operation, and the regenerated liver was weighed on the 0, 3 and 6 days after operation to detect the liver proliferation, and was further judged by the hepatocyte regeneration by Ki-67 staining.
Result:After 4 weeks of CCl₄ and ethanol modeling, the weight of rats increased slowly, their life was basically unaffected, and liver fibrosis was uniform. One rat died due to the injection of CCl₄, and the success rate of liver fibrosis modeling was 95.0%. Liver fibrosis group and control group achieved rapid growth of residual liver volume after ALPPS, and there was no significant difference between the two groups. Ki-67 positive cells in hepatic fibrosis group and normal group increased significantly on the 3rd day after operation and decreased on the 6th day after operation.
Conclusion:Using the method of CCl₄ back subcutaneous injection and drinking ethanol, the liver fibrosis rat model can be established quickly. ALPPS operation is safe and feasible in this fibrosis model. In this study, a stable and efficient ALPPS rat model with liver fibrosis was established.

Key words: Liver fibrosis, Associating liver partition and portal vein ligation for staged hepatectomy, Carbon tetrachloride, Ethanol, Rat

Zhang Junwei, Yang Xu, Wang Yunchao, Wang Yanyu, Xun Ziyu, Cheng Quancheng, Fang Jinyu, Ding Huiru, Meng Jieyi, Zhang Lei, Xu Yiyao, Sang Xinting, Zhao Haitao, Zhang Weiguang, Lu Xin. Establishment of a rat model: associating liver partition and portal vein ligation for staged hepatectomy with hepatic fibrosis[J]. Electronic Journal of Liver Tumor, 2022, 9(2): 1-6.

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