The overexpression of miRNA-10a promotes liver regeneration in rats with liver cirrhosis after hepatectomy

Abstract

Abstract: Objective: To investigate the effect of miRNA-10a on liver regeneration in rats after liver resection in liver cirrhosis.
Methods: Thirty-six cirrhotic rats were randomly divided into a sham operation group (Group A, n=12), a 70% liver resection without transfection group (Group B, n=12), and a 70% liver resection with transfection group (Group C, n=12). Two weeks before surgery, successful hepatocyte-specific transfection of miRNA-10a was performed in rats of Group C. At 12h, 24h, 48h, and 72h after surgery, 3 rats from each group were euthanized, and blood samples from the portal vein were collected for testing serum liver function indicators including alanine aminotransferase (ALT) and albumin (ALB). The levels of miRNA-10a in liver tissue were detected using PCR, and the liver-to-body weight ratio was calculated at different time points.
Results: At 12 h, 24 h, 48 h, and 72 h after surgery, the levels of miRNA-10a in liver tissue of Group C rats were significantly higher than the other two groups, indicating successful overexpression of miRNA-10a. At 12 h, 24 h, 48 h, and 72 h after surgery, the serum ALT levels of Group C rats were lower on average than Group B, while the ALB levels were higher on average than Group B, suggesting that miRNA-10a is beneficial for protecting liver function. At 48 h and 72 h after surgery, the liver-to-body weight ratio of Group C rats was significantly higher than Group B, indicating that overexpression of miRNA-10a promotes liver tissue growth.
Conclusion: Overexpression of miRNA-10a promotes liver regeneration in rats with liver cirrhosis after hepatectomy.

Key words: Hepatocellular carcinoma, Liver regeneration, miRNA-10a, Rat

Yu Zhencen, Gu Xiaohui, Liu Chao, Wang Xin. The overexpression of miRNA-10a promotes liver regeneration in rats with liver cirrhosis after hepatectomy[J]. Electronic Journal of Liver Tumor, 2024, 11(2): 48-52.

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