花姜酮纳米颗粒通过p53通路诱导细胞周期阻滞发挥对肝细胞癌细胞的抗肿瘤作用

肝癌电子杂志 ›› 2020, Vol. 7 ›› Issue (2): 44-47.

花姜酮纳米颗粒通过p53通路诱导细胞周期阻滞发挥对肝细胞癌细胞的抗肿瘤作用

刘海石¹, 张文林², 王鹏¹, 郭佐铭¹, 张玉宝^1,*, 张松岩^1,*

1 哈尔滨医科大学附属肿瘤医院肝胆胰外科,黑龙江哈尔滨 150040;
2 伊春林业管理局中心医院 ,黑龙江伊春153000

收稿日期:2020-04-17 出版日期:2020-06-30 发布日期:2020-07-23
通讯作者: ^* 张松岩 E-mail: zhangsong-yan@163.com;张玉宝 E-mail: Zhyb88077@sina.com
作者简介:刘海石,硕士研究生,哈尔滨医科大学附属肿瘤医院肝胆胰外科。
基金资助:
黑龙江省卫生计生委科研课题（2018-239）

Zerumbone nanoparticles induce cell cycle arrest through the p53 pathway and exert antitumor effects on hepatocellular carcinoma cells

Liu Haishi¹, Zhang Wenlin², Wang Peng¹, Guo Zuoming¹, Zhang Yubao^1,*, Zhang Songyan^1,*

1 Department of Hepatobiliary and Pancreatic Surgery, Harbin Medical University Cancer Hospital, Harbin 150040, Heilongjiang, China;
2 Yichun Forestry Administration Central Hospital , Yichun 153000, Heilongjiang, China

Received:2020-04-17 Online:2020-06-30 Published:2020-07-23

摘要/Abstract

摘要： 目的: 本研究旨在探索花姜酮纳米颗粒对人肝细胞癌细胞抗肿瘤作用及其机制。方法: 检测花姜酮纳米颗粒对人肝细胞癌HepG-2细胞的细胞增殖、集落形成、侵袭、p16、p21、p53、CyclinD1及CDK4蛋白在Western-Blot上的表达水平及细胞周期分布的影响。结果: 花姜酮纳米颗粒可以上调人肝细胞癌HepG-2细胞p16、p21及p53蛋白的表达,下调CyclinD1和CDK4蛋白的表达,引起细胞周期G1-S期阻滞,使人肝细胞癌HepG-2细胞的增殖,集落形成及侵袭能力下降。结论: 花姜酮纳米颗粒通过p53通路引起人肝细胞癌HepG-2 细胞周期G1-S期阻滞,抑制其恶性生物学行为。

关键词: 花姜酮, 纳米, 细胞周期, p53蛋白

Abstract: Objective: The purpose of this study was to explore the anti-tumor effect of HJT1 - NP on human hepatocellular carcinoma cells and its mechanism.Methods: To investigate HJT1-NP effects on cell proliferation, colony formation, invasion, proteins expression levels of p16, p21, p53, CyclinD1 and CDK4 in human hepatocellular carcinoma HepG-2 cells by western-blot analysis and cell cycle distribution.Results: The HJT1 - NP could up-regulate the proteins expression of p16, p21 and p53 in human hepatocellular carcinoma HepG-2 cells, down-regulate the proteins expression of CyclinD1 and CDK4, induce G1-S phase arrest of cell cycle, and decrease the proliferation, colony formation and invasion ability of human hepatocellular carcinoma HepG-2 cells.Conclusion: HJT1-NP could induce G1-S phase arrest of human hepatocellular carcinoma HepG-2 cells and inhibit its malignant biological behavior through P53 pathway.

Key words: Zerumbone, Nanometer, Cell cycle, P53

刘海石, 张文林, 王鹏, 郭佐铭, 张玉宝, 张松岩. 花姜酮纳米颗粒通过p53通路诱导细胞周期阻滞发挥对肝细胞癌细胞的抗肿瘤作用[J]. 肝癌电子杂志, 2020, 7(2): 44-47.

Liu Haishi, Zhang Wenlin, Wang Peng, Guo Zuoming, Zhang Yubao, Zhang Songyan. Zerumbone nanoparticles induce cell cycle arrest through the p53 pathway and exert antitumor effects on hepatocellular carcinoma cells[J]. Electronic Journal of Liver Tumor, 2020, 7(2): 44-47.