Study on the expression and prognosis of miR-320a and GP73 in patients with cholangiocarcinoma

Abstract

Abstract: Objective: To observe the synergistic effect and prognosis of microRNA-320a (miR-320a) and Golgi protein 73 (GP73) in patients with cholangiocarcinoma.Methods: 142 patients with cholangiocarcinoma admitted to our hospital from June 2014 to December 2018 were selected as cholangiocarcinoma group, and 146 cases of physical examination were selected as healthy group during the same period. The expression level of serum miR-320a in the two groups was detected real-time fluorescence quantitative assay (qRT-PCR); the expression level of GP73 in the two groups was determined by enzyme-linked immunosorbent assay (ELISA); the relationships between the expression levels of serum miR-320a, GP73 with clinical characteristics of cholangiocarcinoma and the relationship between expression levels of miR-320a and GP73 were analyzed; the prognostic factors of cholangiocarcinoma were analyzed; and the diagnostic values of levels of serum miR-320a and GP73 in cholangiocarcinoma were analyzed.Results: The expression level of serum miR-320a in cholangiocarcinoma group was significantly lower than that in healthy group (P < 0.05), and the level of GP73 was significantly higher than that in healthy group (P < 0.05); the expression levels of serum miR-320a and GP73 in cholangiocarcinoma group were correlated with vascular invasion, TNM stage and cirrhosis (all P< 0.05); the expression level of miR-320a was negatively correlated with GP73 in patients with cholangiocarcinoma (P< 0.05); vascular invasion, TNM stage, cirrhosis and GP73 were risk factors for the prognosis of cholangiocarcinoma (P< 0.05), and miR-320a was protective factor for the prognosis of cholangiocarcinoma (P< 0.05); the areas under curve (AUC) of the expression levels of serum miR-320a and GP73 in diagnosis of cholangiocarcinoma were 0.875 and 0.878, respectively, the corresponding sensitivities were 84.5% and 85.4% respectively, and the specificities were 79.5% and 79.5% respectively; the AUC of combined diagnosis of serum miR-320a and GP73 for cholangiocarcinoma was 0.956, and the sensitivity and specificity were 90.8% and 91.8% respectively.Conclusions: The expression of serum miR-320a in patients with cholangiocarcinoma is low, and GP73 is high, there is a negative correlation between miR-320a and GP73, and the interaction between them may affect the occurrence and development of cholangiocarcinoma.

Key words: MicroRNA-320a, Golgi protein 73, Cholangiocarcinoma, Expression relationship, Prognosis

Zhang Lei, Sun Jingwu, Zhang Jiaxing. Study on the expression and prognosis of miR-320a and GP73 in patients with cholangiocarcinoma[J]. Electronic Journal of Liver Tumor, 2020, 7(2): 16-20.

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