Electronic Journal of Liver Tumor ›› 2020, Vol. 7 ›› Issue (2): 44-47.

• Original article • Previous Articles     Next Articles

Zerumbone nanoparticles induce cell cycle arrest through the p53 pathway and exert antitumor effects on hepatocellular carcinoma cells

Liu Haishi1, Zhang Wenlin2, Wang Peng1, Guo Zuoming1, Zhang Yubao1,*, Zhang Songyan1,*   

  1. 1 Department of Hepatobiliary and Pancreatic Surgery, Harbin Medical University Cancer Hospital, Harbin 150040, Heilongjiang, China;
    2 Yichun Forestry Administration Central Hospital , Yichun 153000, Heilongjiang, China
  • Received:2020-04-17 Online:2020-06-30 Published:2020-07-23

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Abstract: Objective: The purpose of this study was to explore the anti-tumor effect of HJT1 - NP on human hepatocellular carcinoma cells and its mechanism.Methods: To investigate HJT1-NP effects on cell proliferation, colony formation, invasion, proteins expression levels of p16, p21, p53, CyclinD1 and CDK4 in human hepatocellular carcinoma HepG-2 cells by western-blot analysis and cell cycle distribution.Results: The HJT1 - NP could up-regulate the proteins expression of p16, p21 and p53 in human hepatocellular carcinoma HepG-2 cells, down-regulate the proteins expression of CyclinD1 and CDK4, induce G1-S phase arrest of cell cycle, and decrease the proliferation, colony formation and invasion ability of human hepatocellular carcinoma HepG-2 cells.Conclusion: HJT1-NP could induce G1-S phase arrest of human hepatocellular carcinoma HepG-2 cells and inhibit its malignant biological behavior through P53 pathway.

Key words: Zerumbone, Nanometer, Cell cycle, P53