基于CRISPR/Cas9技术的TRPS1基因敲除小鼠模型的构建

肝癌电子杂志 ›› 2020, Vol. 7 ›› Issue (4): 38-43.

基于CRISPR/Cas9技术的TRPS1基因敲除小鼠模型的构建

李腾雁, 刘文杰, 赵宏, 蔡建强^*

国家癌症中心/ 国家肿瘤临床医学研究中心/ 中国医学科学院北京协和医学院肿瘤医院肝胆外科,北京 100021

收稿日期:2020-09-23 发布日期:2021-02-02
通讯作者: *蔡建强caijianqiang188@sina.com
作者简介:李腾雁,博士研究生,中国医学科学院北京协和医学院肿瘤医院,肝胆外科
基金资助:
国家自然科学基金(81672461); 国家自然科学基金(81972311); 深圳市“医疗卫生三名工程”（SZSM202011010）首都卫生发展科研专项项目(2018-1-4021); 中国医学科学院医学与健康科技创新工程(2016-I2M-1-001,2017-12M-4-002)

Generation of TRPS1 knockout mice by CRISPR/Cas9-mediated gene targeting

Li Tengyan, Liu Wenjie, Zhao Hong, Cai Jianqiang^*

National Department of Hepatobiliary Surgery, National Cancer Center/National Clinical Research Center for Cancer/ Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China

Received:2020-09-23 Published:2021-02-02

摘要/Abstract

摘要： 目的：基于CRISPR/Cas9技术构建敲除TRPS1基因的杂合子小鼠,并进行鉴定。方法： C57BL/6N小鼠自行交配后,使用Cas9/sgRNA注射受精卵的方法构建基因敲除小鼠,对可遗传的小鼠基因型进行鼠尾检测,TRPS1杂合子敲除小鼠分别与野生型小鼠交配,获得具有稳定基因型的小鼠。结果：本实验通过使用Cas9/sgRNA注射受精卵的方法,所有繁殖小鼠经鼠尾基因型鉴定,证实成功构建了18只TRPS1基因敲除的杂合子小鼠。结论：基于CRISPR/Cas9技术成功构建了敲除TRPS1基因的杂合子小鼠。

关键词: CRISPR/Cas9, TRPS1, 结直肠癌, 基因敲除小鼠

Abstract: Objectives: This study aimed to construct and identify heterozygous mice knocked out of TRPS1 gene based on CRISPR/Cas9 technology. Methods: After self-mating of C57BL/6N mice, TRPS1 knockout mice were constructed by injecting fertilized eggs with Cas9/sgRNA, and the mouse genotypes of heritable mice were detected by tail. TRPS1 heterozygous knockout mice were mated with wild-type mice to obtain mice with stable genotypes. Results: In this experiment, the fertilized eggs were injected with cas9 / sgRNA, all breeding mice were identified by tail genotype, 18 TRPS1 knockout heterozygous mice were successfully constructed. Conclusion: In this study, we successfully constructed TRPS1 knockout heterozygous mice based on CRISPR / cas9 technology, which provided a research platform for further research on the role of TRPS1 in the occurrence, development and possible liver metastasis of colorectal cancer at the animal level.

Key words: CRISPR/Cas9, TRPS1, Colorectal cancer, Gene knockout mouse

李腾雁, 刘文杰, 赵宏, 蔡建强. 基于CRISPR/Cas9技术的TRPS1基因敲除小鼠模型的构建[J]. 肝癌电子杂志, 2020, 7(4): 38-43.

Li Tengyan, Liu Wenjie, Zhao Hong, Cai Jianqiang. Generation of TRPS1 knockout mice by CRISPR/Cas9-mediated gene targeting[J]. Electronic Journal of Liver Tumor, 2020, 7(4): 38-43.

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